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KMID : 1007020070050020100
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Korean Soceity of Osteroporosis
2007 Volume.5 No. 2 p.100 ~ p.111
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Study on the Adult Human Mesenchymal Stem Cell-mediated Osteogenesis on the Bone Graft Matrix
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Park Joong-Yeop
Kim Tak
Rim Jae-Suk
Lee Eui-Seok
Jang Hyon-Seok
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Abstract
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Objectives: Tissue engineering demands the interaction of engineering and life sciences to generate biologic substitutes that can create, preserve or restore lost organ function. Functional tissue engineering of bone tissue generally involves the use of differentiated or progenitor cells seeded with specific growth factors in biomaterial scaffolds. The purpose of this study was to compare the osteogenic capacity of bone marrow-derived, culture expanded human mesenchymal stem cell seeded to 3 different scaffolds.
Methods: Cells were loaded onto 2×2 mm cubes of synthetic HA/TCP, anorganic bovine bone (Bio-Ossⓡ) and coralline-derived HA (ProOsteonⓡ) and placed into the dorsal subcutaneous pocket of SCID mouse for 5 weeks.
Results: Synthetic HA/TCP was the most effective bone forming scaffold compared with Bio-Ossⓡ and ProOsteonⓡ. Scanning electron microscopy evaluation of loaded scaffolds indicates that cell loading was not a variable affecting the different bone formation outcomes in these 3 scaffolds.
Conclusion: Synthetic HA/TCP can be used as the efficient scaffold, compared to Bio-Ossⓡ and ProOsteonⓡ.
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KEYWORD
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Bone tissue engineering, Human emsenchymal stem cell, Osteogenic capacity
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